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cd45 rabbit 20103 1 ap proteintech  (Proteintech)


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    Structured Review

    Proteintech cd45 rabbit 20103 1 ap proteintech
    Cd45 Rabbit 20103 1 Ap Proteintech, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd45+rabbit+20103+1+ap+proteintech/CD45+Antibody/pmc12220947__jciinsight-10-187424-s206-11-124-127
    Average 96 stars, based on 235 article reviews
    cd45 rabbit 20103 1 ap proteintech - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Immunocytochemistry:

    Article Title: Type 2 diabetes alters quiescent pancreatic stellate cells to tumor-prone state
    Article Snippet: Vendor Experiments Dilutions M6a Mouse D055-3 MBL Life Science (Tokyo, Japan) ICC 1:200 PTN Rabbit 27117-1-AP Proteintech (IL, USA) ICC 1:200 PDGFRα Rabbit EPR22059-270 Abcam (Cambridge, UK) ICC 1:200 Sca1 Rat E13 161-7 Abcam (Cambridge, UK) IHC, ICC 1:1000 CXCL13 Goat AF472 R&D systems (MN, USA) IHC, ICC, IF 1:100 Ly-6c Rat HK1.4 BioLegend (CA, USA) IHC, ICC 1:100 SMA Rabbit EPR5368 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 FABP4 Rabbit EPR3579 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 CD31 Rabbit D8V9E Cell Signaling Technology, Inc., (MA, USA) IHC 1:100 Ki67 Rabbit ab66155 Abcam (Cambridge, UK) IHC 1:200 CD3 Mouse F7.2.38 Agilent (CA, USA) IHC 1:200 CD8 Mouse C8/144B Agilent (CA, USA) IHC 1:200 B220 Rat RA3-6B2 (RUO) BD Bioscience (NJ, USA) IHC 1:200 CD45 Rabbit 20103-1-AP Proteintech (IL, USA) IHC 1:200 CD20 Mouse L26 Leica (Wetzlar, Germany) IHC 1:200 CXCL13 Goat AF801 R&D systems (MN, USA) IHC 1:100 F4/80-PE/cy7 Rat BM8 BioLegend (CA, USA) FACS 1:100 CD326 (Ep-CAM)-FITC Rat G8.8 BioLegend (CA, USA) FACS 1:100 Ly6c-FITC Rat HK1.4 BioLegend (CA, USA) FACS 1:50 Sca1-PE Rat D7 BioLegend (CA, USA) FACS 1:200 ICC, immunocytochemistry: IHC, immunohistochemistry: FACS, fluorescence assisted cell sorting: IF, immunofluorescence.

    Immunohistochemistry:

    Article Title: Type 2 diabetes alters quiescent pancreatic stellate cells to tumor-prone state
    Article Snippet: Vendor Experiments Dilutions M6a Mouse D055-3 MBL Life Science (Tokyo, Japan) ICC 1:200 PTN Rabbit 27117-1-AP Proteintech (IL, USA) ICC 1:200 PDGFRα Rabbit EPR22059-270 Abcam (Cambridge, UK) ICC 1:200 Sca1 Rat E13 161-7 Abcam (Cambridge, UK) IHC, ICC 1:1000 CXCL13 Goat AF472 R&D systems (MN, USA) IHC, ICC, IF 1:100 Ly-6c Rat HK1.4 BioLegend (CA, USA) IHC, ICC 1:100 SMA Rabbit EPR5368 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 FABP4 Rabbit EPR3579 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 CD31 Rabbit D8V9E Cell Signaling Technology, Inc., (MA, USA) IHC 1:100 Ki67 Rabbit ab66155 Abcam (Cambridge, UK) IHC 1:200 CD3 Mouse F7.2.38 Agilent (CA, USA) IHC 1:200 CD8 Mouse C8/144B Agilent (CA, USA) IHC 1:200 B220 Rat RA3-6B2 (RUO) BD Bioscience (NJ, USA) IHC 1:200 CD45 Rabbit 20103-1-AP Proteintech (IL, USA) IHC 1:200 CD20 Mouse L26 Leica (Wetzlar, Germany) IHC 1:200 CXCL13 Goat AF801 R&D systems (MN, USA) IHC 1:100 F4/80-PE/cy7 Rat BM8 BioLegend (CA, USA) FACS 1:100 CD326 (Ep-CAM)-FITC Rat G8.8 BioLegend (CA, USA) FACS 1:100 Ly6c-FITC Rat HK1.4 BioLegend (CA, USA) FACS 1:50 Sca1-PE Rat D7 BioLegend (CA, USA) FACS 1:200 ICC, immunocytochemistry: IHC, immunohistochemistry: FACS, fluorescence assisted cell sorting: IF, immunofluorescence.

    FACS:

    Article Title: Type 2 diabetes alters quiescent pancreatic stellate cells to tumor-prone state
    Article Snippet: Vendor Experiments Dilutions M6a Mouse D055-3 MBL Life Science (Tokyo, Japan) ICC 1:200 PTN Rabbit 27117-1-AP Proteintech (IL, USA) ICC 1:200 PDGFRα Rabbit EPR22059-270 Abcam (Cambridge, UK) ICC 1:200 Sca1 Rat E13 161-7 Abcam (Cambridge, UK) IHC, ICC 1:1000 CXCL13 Goat AF472 R&D systems (MN, USA) IHC, ICC, IF 1:100 Ly-6c Rat HK1.4 BioLegend (CA, USA) IHC, ICC 1:100 SMA Rabbit EPR5368 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 FABP4 Rabbit EPR3579 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 CD31 Rabbit D8V9E Cell Signaling Technology, Inc., (MA, USA) IHC 1:100 Ki67 Rabbit ab66155 Abcam (Cambridge, UK) IHC 1:200 CD3 Mouse F7.2.38 Agilent (CA, USA) IHC 1:200 CD8 Mouse C8/144B Agilent (CA, USA) IHC 1:200 B220 Rat RA3-6B2 (RUO) BD Bioscience (NJ, USA) IHC 1:200 CD45 Rabbit 20103-1-AP Proteintech (IL, USA) IHC 1:200 CD20 Mouse L26 Leica (Wetzlar, Germany) IHC 1:200 CXCL13 Goat AF801 R&D systems (MN, USA) IHC 1:100 F4/80-PE/cy7 Rat BM8 BioLegend (CA, USA) FACS 1:100 CD326 (Ep-CAM)-FITC Rat G8.8 BioLegend (CA, USA) FACS 1:100 Ly6c-FITC Rat HK1.4 BioLegend (CA, USA) FACS 1:50 Sca1-PE Rat D7 BioLegend (CA, USA) FACS 1:200 ICC, immunocytochemistry: IHC, immunohistochemistry: FACS, fluorescence assisted cell sorting: IF, immunofluorescence.

    Fluorescence:

    Article Title: Type 2 diabetes alters quiescent pancreatic stellate cells to tumor-prone state
    Article Snippet: Vendor Experiments Dilutions M6a Mouse D055-3 MBL Life Science (Tokyo, Japan) ICC 1:200 PTN Rabbit 27117-1-AP Proteintech (IL, USA) ICC 1:200 PDGFRα Rabbit EPR22059-270 Abcam (Cambridge, UK) ICC 1:200 Sca1 Rat E13 161-7 Abcam (Cambridge, UK) IHC, ICC 1:1000 CXCL13 Goat AF472 R&D systems (MN, USA) IHC, ICC, IF 1:100 Ly-6c Rat HK1.4 BioLegend (CA, USA) IHC, ICC 1:100 SMA Rabbit EPR5368 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 FABP4 Rabbit EPR3579 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 CD31 Rabbit D8V9E Cell Signaling Technology, Inc., (MA, USA) IHC 1:100 Ki67 Rabbit ab66155 Abcam (Cambridge, UK) IHC 1:200 CD3 Mouse F7.2.38 Agilent (CA, USA) IHC 1:200 CD8 Mouse C8/144B Agilent (CA, USA) IHC 1:200 B220 Rat RA3-6B2 (RUO) BD Bioscience (NJ, USA) IHC 1:200 CD45 Rabbit 20103-1-AP Proteintech (IL, USA) IHC 1:200 CD20 Mouse L26 Leica (Wetzlar, Germany) IHC 1:200 CXCL13 Goat AF801 R&D systems (MN, USA) IHC 1:100 F4/80-PE/cy7 Rat BM8 BioLegend (CA, USA) FACS 1:100 CD326 (Ep-CAM)-FITC Rat G8.8 BioLegend (CA, USA) FACS 1:100 Ly6c-FITC Rat HK1.4 BioLegend (CA, USA) FACS 1:50 Sca1-PE Rat D7 BioLegend (CA, USA) FACS 1:200 ICC, immunocytochemistry: IHC, immunohistochemistry: FACS, fluorescence assisted cell sorting: IF, immunofluorescence.

    Immunofluorescence:

    Article Title: Type 2 diabetes alters quiescent pancreatic stellate cells to tumor-prone state
    Article Snippet: Vendor Experiments Dilutions M6a Mouse D055-3 MBL Life Science (Tokyo, Japan) ICC 1:200 PTN Rabbit 27117-1-AP Proteintech (IL, USA) ICC 1:200 PDGFRα Rabbit EPR22059-270 Abcam (Cambridge, UK) ICC 1:200 Sca1 Rat E13 161-7 Abcam (Cambridge, UK) IHC, ICC 1:1000 CXCL13 Goat AF472 R&D systems (MN, USA) IHC, ICC, IF 1:100 Ly-6c Rat HK1.4 BioLegend (CA, USA) IHC, ICC 1:100 SMA Rabbit EPR5368 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 FABP4 Rabbit EPR3579 Abcam (Cambridge, UK) IHC, ICC, IF 1:200 CD31 Rabbit D8V9E Cell Signaling Technology, Inc., (MA, USA) IHC 1:100 Ki67 Rabbit ab66155 Abcam (Cambridge, UK) IHC 1:200 CD3 Mouse F7.2.38 Agilent (CA, USA) IHC 1:200 CD8 Mouse C8/144B Agilent (CA, USA) IHC 1:200 B220 Rat RA3-6B2 (RUO) BD Bioscience (NJ, USA) IHC 1:200 CD45 Rabbit 20103-1-AP Proteintech (IL, USA) IHC 1:200 CD20 Mouse L26 Leica (Wetzlar, Germany) IHC 1:200 CXCL13 Goat AF801 R&D systems (MN, USA) IHC 1:100 F4/80-PE/cy7 Rat BM8 BioLegend (CA, USA) FACS 1:100 CD326 (Ep-CAM)-FITC Rat G8.8 BioLegend (CA, USA) FACS 1:100 Ly6c-FITC Rat HK1.4 BioLegend (CA, USA) FACS 1:50 Sca1-PE Rat D7 BioLegend (CA, USA) FACS 1:200 ICC, immunocytochemistry: IHC, immunohistochemistry: FACS, fluorescence assisted cell sorting: IF, immunofluorescence.



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    Proteintech sa00007 7 cd45 rabbit polyclonal antibody proteintech 20103 1 ap fitc anti mouse cd326 ep cam
    Figure 4. Specific genes related to hypoxia stimulation and platelet activation were enriched in the CTM group versus the control group (A) Images of CTM released from the Cluster-Chip and live stained with FITC anti-mouse <t>CD326</t> (EPCAM) (green) and Alex594-conjugated antibodies against <t>CD45</t> (red). (B) Heatmap of gene expression related to epithelial status, mesenchymal status, cell stemness, leukocytes, cell junctions, and platelets in samples from each group. (C) Gene Ontology enrichment analysis in treatment vs. control groups. (D) Heatmap of gene expression associated with CTM-mediating metastasis in the two groups of samples, with some genes marked with red and some marked with green relating to hypoxia stimulation and platelet activation, respectively. (E) Verification of key genes that are differently expressed in treatment vs. control group by qPCR. (F) Representative fluorescent micrographs of 4T1 cell clusters captured on the chip following culture in ultra-low adhesion well plates with or without platelets under the hypoxic or normoxic conditions. CTC-clusters stained for Pan-cytokeratin and EPCAM (green), P-selectin (red), and DAPI (nuclei, blue). Scale bar: 50 mm and 20 mm. (G) The statistic quantification of the different types of captured CTM cultured in 4 conditions as mentioned in (F). Data were presented as mean G SD. *0.01 < p % 0.05; **0.001 < p % 0.01; ***p % 0.001.
    Sa00007 7 Cd45 Rabbit Polyclonal Antibody Proteintech 20103 1 Ap Fitc Anti Mouse Cd326 Ep Cam, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Figure 4. Specific genes related to hypoxia stimulation and platelet activation were enriched in the CTM group versus the control group (A) Images of CTM released from the Cluster-Chip and live stained with FITC anti-mouse CD326 (EPCAM) (green) and Alex594-conjugated antibodies against CD45 (red). (B) Heatmap of gene expression related to epithelial status, mesenchymal status, cell stemness, leukocytes, cell junctions, and platelets in samples from each group. (C) Gene Ontology enrichment analysis in treatment vs. control groups. (D) Heatmap of gene expression associated with CTM-mediating metastasis in the two groups of samples, with some genes marked with red and some marked with green relating to hypoxia stimulation and platelet activation, respectively. (E) Verification of key genes that are differently expressed in treatment vs. control group by qPCR. (F) Representative fluorescent micrographs of 4T1 cell clusters captured on the chip following culture in ultra-low adhesion well plates with or without platelets under the hypoxic or normoxic conditions. CTC-clusters stained for Pan-cytokeratin and EPCAM (green), P-selectin (red), and DAPI (nuclei, blue). Scale bar: 50 mm and 20 mm. (G) The statistic quantification of the different types of captured CTM cultured in 4 conditions as mentioned in (F). Data were presented as mean G SD. *0.01 < p % 0.05; **0.001 < p % 0.01; ***p % 0.001.

    Journal: iScience

    Article Title: Hypoxia stimulates CTC-platelet cluster formation to promote breast cancer metastasis.

    doi: 10.1016/j.isci.2024.109547

    Figure Lengend Snippet: Figure 4. Specific genes related to hypoxia stimulation and platelet activation were enriched in the CTM group versus the control group (A) Images of CTM released from the Cluster-Chip and live stained with FITC anti-mouse CD326 (EPCAM) (green) and Alex594-conjugated antibodies against CD45 (red). (B) Heatmap of gene expression related to epithelial status, mesenchymal status, cell stemness, leukocytes, cell junctions, and platelets in samples from each group. (C) Gene Ontology enrichment analysis in treatment vs. control groups. (D) Heatmap of gene expression associated with CTM-mediating metastasis in the two groups of samples, with some genes marked with red and some marked with green relating to hypoxia stimulation and platelet activation, respectively. (E) Verification of key genes that are differently expressed in treatment vs. control group by qPCR. (F) Representative fluorescent micrographs of 4T1 cell clusters captured on the chip following culture in ultra-low adhesion well plates with or without platelets under the hypoxic or normoxic conditions. CTC-clusters stained for Pan-cytokeratin and EPCAM (green), P-selectin (red), and DAPI (nuclei, blue). Scale bar: 50 mm and 20 mm. (G) The statistic quantification of the different types of captured CTM cultured in 4 conditions as mentioned in (F). Data were presented as mean G SD. *0.01 < p % 0.05; **0.001 < p % 0.01; ***p % 0.001.

    Article Snippet: Antibodies Anti-pan cytokeratin Abcam ab7753 P-Selectin polyclonal antibody Proteintech 13304-1-AP CD45R (RA3-6B2) Santa Cruz sc-19597 Alexa Fluor 488-labeled goat anti-mouse lgG (H + L) Proteintech SA00013-1 594-labeled goat anti-rabbit lgG (H + L) Proteintech SA00013-4 TRITIC-labeled goat anti-Rat lgG (H + L) Proteintech SA00007-7 CD45 Rabbit Polyclonal antibody Proteintech 20103-1-AP FITC anti-mouse CD326 (Ep-CAM) Biolegend 118207 Alex 594 anti-mouse CD45 Biolegend 103144 Chemicals, peptides, and recombinant proteins Fetal bovine serum ExCell Bio FSP500 RPMI-1640 medium HyClone SH30027.02

    Techniques: Activation Assay, Control, Staining, Gene Expression, Cell Culture